Efficient production of 4-acetylantroquinonol B in submerged cultures of Antrodia cinnamomea via addition of Chinese herbal medicine extracts

Antrodia cinnamomea is a valuable fungus. The 4-acetylantroquinonol B (4-AAQB) contained in it has strong anticancer activity. In this study, five kinds of herbs, burdock, wolfberry, coix seed, hawthorn, and tangerine peel were selected processed into powders, aqueous extracts, ethanolic extracts to investigate the effects on production 4-AAQB by A. cinnamomea. A combination strategy was conducted adding burdock extract. By means, improved 54.5 mg/L, which approximately 33-fold higher than control. After analysis components extract addition major components, vanillic acid, protocatechuic their analogs played an important role synthesis 4-AAQB. It demonstrated that Chinese herbs facilitated both cell growth production, providing feasible way increase yield